Journal: International journal of molecular sciences

Article Title: Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps.

doi: 10.3390/ijms23031018

Figure Lengend Snippet: Figure 5. The pivotal effect of PM10-induced IL-33 for Th2 differentiation effects of conditioned media on PM10-induced human nasal polyp derived fibroblast (NPDFs-CM) on the inflammatory cytokine production in CD4+ T cells. (A) Th2 differentiation was determined by the expression of GATA3 and ST2 (IL-33 receptor). (B,C) The protein levels of IFN-γ, IL-17A, IL-4, IL-13, and IL-10 in CD4+ T cell populations were determined by flow cytometry according to the addition of ST2. The graphic data represents the means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.0001 as compared to the PM10-treated group.

Article Snippet: TMCs (1 × 106 cells) were cultured with 1 μg/mL of phytohemagglutinin (PHA) in NPDF-CM for 72 h. For Th2 differentiation, CD4+ T cells from TMCs were purified using a CD4+ T Cell Isolation Kit MicroBeads (Miltenyi Biotech, Bisley, Surrey, UK), and purified CD4+ T cells were differentiated using a CellXVivo Human Th2 Cell Differentiation Kit (RnDSystems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Derivative Assay, Expressing, Cytometry

Journal: Molecular Genetics & Genomic Medicine

Article Title: Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation

doi: 10.1002/mgg3.1538

Figure Lengend Snippet: Associations between TCF7, MAP3K1, ENTPD1, NT5E, and 55 genes involved in autoimmune diseases and 116Th17 differentiation genes. TCF7, MAP3K1, ENTPD1, and NT5E are strongly associated to Th17 differentiation genes shown in purple. Additionally, there are a large number of interactions between selected genes and autoimmune disease‐relevant genes depicted in blue.

Article Snippet: The culture plates were coated overnight with 5 μg/ml anti‐CD3 antibody (R&D Systems) at 2‐8°C, and then, cells were cultured under Th17 cell‐polarizing condition using the CellXVivo Human Th17 Cell Differentiation Kit (R&D Systems) at 37°C in a humidified atmosphere containing 5% CO 2 for 6 days (Hakemi et al., , ).

Techniques:

Journal: Molecular Genetics & Genomic Medicine

Article Title: Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation

doi: 10.1002/mgg3.1538

Figure Lengend Snippet: Heatmap of miR‐9‐5p and miR‐193b‐3p related signaling pathways. (a) miR‐9‐5p involvement in multiple Th17 differentiation pathways such as TGF‐β signaling, NF‐kappa B signaling, MAPK signaling, HIF‐1 signaling. (b) Association of miR‐193b with critical pathways regulating Th17 differentiation including Toll‐like receptor, and Notch signaling pathways. Color gradient demonstrates pathway values with pale yellow, as the lowest and red, as the highest importance.

Article Snippet: The culture plates were coated overnight with 5 μg/ml anti‐CD3 antibody (R&D Systems) at 2‐8°C, and then, cells were cultured under Th17 cell‐polarizing condition using the CellXVivo Human Th17 Cell Differentiation Kit (R&D Systems) at 37°C in a humidified atmosphere containing 5% CO 2 for 6 days (Hakemi et al., , ).

Techniques: Protein-Protein interactions

Journal: Molecular Genetics & Genomic Medicine

Article Title: Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation

doi: 10.1002/mgg3.1538

Figure Lengend Snippet: Differentiation of Th17 cells. (a) Representative images of differentiating human naïve CD + T cells on day 0 to 6. The number and size of cellular aggregations increased during differentiation, promoting differentiation process by increasing cell‐cell contacts. (b) Concentration of IL‐17 as a characteristic marker of Th17 cells during differentiation. IL‐17 secretion rose substantially during differentiation, reaching a peak on day 6. (** p < 0.01, *** p < 0.001, students t ‐test; Scale bar = 100 μM).

Article Snippet: The culture plates were coated overnight with 5 μg/ml anti‐CD3 antibody (R&D Systems) at 2‐8°C, and then, cells were cultured under Th17 cell‐polarizing condition using the CellXVivo Human Th17 Cell Differentiation Kit (R&D Systems) at 37°C in a humidified atmosphere containing 5% CO 2 for 6 days (Hakemi et al., , ).

Techniques: Concentration Assay, Marker

Journal: Molecular Genetics & Genomic Medicine

Article Title: Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation

doi: 10.1002/mgg3.1538

Figure Lengend Snippet: RT‐qPCR analysis of TCF7, MAP3K1, ENTPD1, and NT5E expression levels during human Th17 differentiation. The expression levels of TCF7 (a), MAP3K1 (b), ENTPD1 (c), and NT5E (d) declined dramatically during Th17 differentiation. Expression of genes was normalized to GAPDH level as an internal control (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, students t ‐test).

Article Snippet: The culture plates were coated overnight with 5 μg/ml anti‐CD3 antibody (R&D Systems) at 2‐8°C, and then, cells were cultured under Th17 cell‐polarizing condition using the CellXVivo Human Th17 Cell Differentiation Kit (R&D Systems) at 37°C in a humidified atmosphere containing 5% CO 2 for 6 days (Hakemi et al., , ).

Techniques: Quantitative RT-PCR, Expressing, Control

Journal: Molecular Genetics & Genomic Medicine

Article Title: Upregulation of miR‐9 and miR‐193b over human Th17 cell differentiation

doi: 10.1002/mgg3.1538

Figure Lengend Snippet: RT‐qPCR analysis of miR‐9, and miR‐193b expression levels over human Th17 differentiation. (a) miR‐9 expression increased significantly over human Th17 differentiation. (b) There was a substantial rise in miR‐193b level during human Th17 differentiation. Results were normalized to U6 snRNA expression (* p < 0.05, ** p < 0.01, students t ‐test).

Article Snippet: The culture plates were coated overnight with 5 μg/ml anti‐CD3 antibody (R&D Systems) at 2‐8°C, and then, cells were cultured under Th17 cell‐polarizing condition using the CellXVivo Human Th17 Cell Differentiation Kit (R&D Systems) at 37°C in a humidified atmosphere containing 5% CO 2 for 6 days (Hakemi et al., , ).

Techniques: Quantitative RT-PCR, Expressing